APVV-14-0753

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Biochips and biosensors for glycorecognition, their development, preparation and application in cancer research       

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Principal investigator: Jaroslav Katrlik
Principal investigator (IMPG SAS): Zdena Sulova

Duration: July 2015 – June 2018
Coordinating institution: Institute of Chemistry, SAS,Bratislava

Annotation:

Due to the rapid development of new technologies is placed a considerable emphasis on their use for improving the quality of life. The most important areas here include the care of human health, such as the fight against cancer. Here is a keen interest in new sensitive diagnostic approaches and technologies to help increase the effectiveness of therapy and cancer research. Development and application of new diagnostic procedures and methods is based in no small measure on the knowledge gained in the basic research in the field of analytical tools based on the biochips and biosensors. The aim of the project is the development and preparation of new analytical glyco-recognizing biochip and biosensor systems based on lectin in different formats in combination with multiple platforms and innovative detection methods, their validation and use in the analysis of real samples in cancer research. The selected design of biochips and used detection platform enable highly sensitive and high-throughput analysis of glycosylation changes, and the involvement of mass spectrometry also accurate identification of glycan structures subject to glycosylation changes. To increase the functionality of biochips will be in the development of analytical systems used also nanotechnology tools, particularly in the preparation of nanostructured surfaces of biochips and for improving the efficiency of detection. Various types of biological samples will be analyzed such as serum, lysates, tissue and isolated glycoproteins from some areas of cancer research (effect of hypoxia, resistance to cytostatics, search of glyco-biomarkers). It is expected that the involvement of the entire spectrum of analytical procedures for glycorecognition developed in this project will help formulate more sophisticated conclusions on the role of glycans in cancer.

Keywords:

glycosylation, lectin, biochip, biosensor, microarray, graphene, gold nanoparticles, quantum dots, cancer research

Objectives:

  • Design, optimization of the preparation and characterization of new original biochips and biosensors for the glycorecognition for for each detection platform (microarray, EIS, SPR) containing biorecognition elements
  • Optimization of analysis procedures for each platform and configurations of biochips
  • Testing of functional characteristics of developed systems in model and real biological samples, validation and application in determination of glycosylation changes in samples in the context of cancer research, identification of selected glycan structures of glycoproteins and correlation of results

Publications:
PubMed Pavlikova L, Seres M, Imrichova D, Hano M, Rusnak A, Zamorova M, Katrlik J, Breier A, Sulova Z. The expression of P-gp in leukemia cells is associated with cross-resistance to protein N-glycosylation inhibitor tunicamycin. Gen Physiol Biophys. 35 (2016) 497-510